#3419 LINARIN PROTECTS THE KIDNEY FROM ISCHEMIA-REPERFUSION INJURY AND SUPPRESSES ETS2 INDUCED ACUTE KIDNEY INJURY

Abstract Background and Aims Ischemia/reperfusion injury (IRI), a participant in acute kidney (AKI), can occur as series of pathological processes such inflammation. Linarin (LIN) has been widely used for different diseases. To confirm the anti-inflammatory value relevant mechanism LIN during IRI, vivo vitro model were established. Method or isovolumetric DMSO was given, histologic analysis, quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR), serum creatinine (SCr) testing blood urea nitrogen (BUN) to evaluate injury. Microarray protein-protein interaction (PPI) analysis molecular docking identify target protein LIN. Results At first, we find that could inhabit IRI decrease expression IL-12 p40 model. explore LIN, collected raw data from public microarray database identified E26 oncogene homolog 2 (ETS2) according PPI docking. The contact area is highly conserved located on domain ETS2 which indicated may alter with synergistical regulation expression. Conclusion Our study demonstrated effect IRI-triggered AKI, broadening medicinal therapeutic options AKI.